GlaDOS from "Portal"
The snide, sarcastic supercomputer from the hit video game franchise sounds like an auto-tuned Speak & Spell with a head cold. And at first you'd hardly suspect anything was wrong with her except for maybe her attitude. But over time, she reveals herself to be downright misleading and capricious, and the final conflict between the player and GlaDOS's personality core harkens back to the unplugging of HAL. Seems all those behavioral fail-safe systems installed into her to keep her from killing anyone only made her find cleverer ways to circumvent them.
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